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Question
Chemistry
Posted 6 months ago
(8(8 Place the following tube types listed below in the approved order of draw (1,2,3(1,2,3 or 4)), as recommended by the manufacturer.
Vacutainer Draw/ Butterfly: EDTA, SST, Sodium Citrate, Fluoride Oxalate
Needle /Syringe Draw: EDTA, SST, Sodium Citrate, Fluoride Oxalate
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Answer from Sia
Posted 6 months ago
Solution
1
Identify the correct order of draw for Vacutainer/Butterfly system: The order of draw is important to prevent cross-contamination of additives between tubes. The recommended order is based on the type of additive and its potential to interfere with tests in subsequent tubes
2
Order for Vacutainer/Butterfly: The correct order for the Vacutainer or Butterfly system, according to the Clinical and Laboratory Standards Institute (CLSI), is as follows: Blood cultures, coagulation tubes (Sodium Citrate), serum tubes with or without clot activator (SST), and then tubes containing additives that could interfere with coagulation tests (EDTA), followed by tubes for glucose testing (Fluoride Oxalate)
3
Identify the correct order of draw for Needle/Syringe system: The order of draw for the Needle/Syringe system is similar to that of the Vacutainer/Butterfly system, with the goal of minimizing contamination and maintaining the integrity of the blood samples
4
Order for Needle/Syringe: The order for the Needle/Syringe system is generally the same as for the Vacutainer/Butterfly system. However, when using a syringe, it is important to transfer the blood to the tubes in the correct order immediately after the draw to avoid clotting and ensure proper mixing of blood with the additives
Answer
Vacutainer Draw/Butterfly: 1. Sodium Citrate, 2. SST, 3. EDTA, 4. Fluoride Oxalate Needle/Syringe Draw: 1. Sodium Citrate, 2. SST, 3. EDTA, 4. Fluoride Oxalate
Key Concept
The order of draw is crucial to prevent cross-contamination and ensure accurate test results.
Explanation
The order is determined by the type of additive in the tube and its potential to interfere with tests in subsequent tubes. Sodium Citrate tubes are drawn first to avoid contamination with other additives that could affect coagulation tests.

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