A $100 \mathrm{~mL}$ volume of tryptone broth is inoculated with $0.5 \mathrm{~mL}$ of overnight culture of $\mathrm{E}$. coli. Following the reading of a sample's absorbance, a serial dilution is then performed by transferring $0.6 \mathrm{~mL}$ from the sample into $9.4 \mathrm{~mL}$ of tryptone broth. That diluted sample is vortexed, and $0.5 \mathrm{~mL}$ is withdrawn and diluted into a second tube, containing $5.5 \mathrm{~mL}$ of trypt

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∻Answer∻ ⚹ The dilution process described involves two steps, resulting in a final dilution factor of 1:220. ⚹ ∻Solution∻ ‖ a ⋮ Initial Dilution: The first dilution involves adding $0.6 \mathrm{~mL}$ of the overnight culture to $9.4 \mathrm{~mL}$ of tryptone broth, creating a dilution factor of $\frac{10}{0.6} = 16.\overline{6}$ or approximately 1:17. ‖ ‖ b ⋮ Second Dilution: The second dilution involves taking $0.5 \mathrm{~mL}$ of the first dilution and adding it to $5.5 \mathrm{~mL}$ of broth, resulting in a dilution factor of $\frac{6}{0.5} = 12$. ‖ ‖ c ⋮ Total Dilution Factor: To find the total dilution factor, multiply the dilution factors of both steps: $16.\overline{6} imes 12 = 200$. Therefore, the overall dilution factor is 1:200. ‖ ∻Key Concept∻ ⚹ Serial dilution is a stepwise dilution of a substance in solution. ⚹ ∻Explanation∻ ⚹ The overall dilution factor is calculated by multiplying the dilution factors of each step in the serial dilution process. ⚹◊What is the purpose of using a vortex mixer in the scientific procedure involving the dilution of a bacterial culture?⍭ Generate me a similar question◊

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