A student measures the absorbance of a solution containing $\mathrm{FeSCN}^{2+}$ ions using a spectrophotometer. The cuvette used by the student has two frosted walls and two transparent walls. The student properly orients the cuvette so that the path of the light goes through the transparent sides of the cuvette when calibrating the spectrophotometer. How will the measured absorbance of the $\mathrm{FeSCN}^{2+}$ be affected if the student incorrectly orients the cuvette so that the path of the light is through the frosted sides of the cuvette?
(A) The measured absorbance of the $\mathrm{FeSCN}^{2+}$ solution will not be affected.

B The measured absorbance of the $\mathrm{FeSCN}^{2+}$ solution will be higher than the actual absorbance.
(C) The measured absorbance of the $\mathrm{FeSCN}^{2+}$ solution will befirower than the actual absorbance.
(D) The effect on the measured absorbance of the $\mathrm{FeSCN}^{2+}$ solution depends on the concentration of the $\mathrm{FeSCN}^{2+}$ solution.

Question

A student measures the absorbance of a solution containing $\mathrm{FeSCN}^{2+}$ ions using a spectrophotometer. The cuvette used by the student has two frosted walls and two transparent walls. The student properly orients the cuvette so that the path of the light goes through the transparent sides of the cuvette when calibrating the spectrophotometer. How will the measured absorbance of the $\mathrm{FeSCN}^{2+}$ be affected if the student incorrectly orients the cuvette so that the path of the light is through the frosted sides of the cuvette?
(A) The measured absorbance of the $\mathrm{FeSCN}^{2+}$ solution will not be affected.

B The measured absorbance of the $\mathrm{FeSCN}^{2+}$ solution will be higher than the actual absorbance.
(C) The measured absorbance of the $\mathrm{FeSCN}^{2+}$ solution will befirower than the actual absorbance.
(D) The effect on the measured absorbance of the $\mathrm{FeSCN}^{2+}$ solution depends on the concentration of the $\mathrm{FeSCN}^{2+}$ solution.

Accepted Answer

***B*** ∻Key Concept∻ ⚹Absorbance and Light Path⚹ ∻Explanation∻ ⚹If the cuvette is oriented incorrectly so that the light passes through the frosted sides, the light will be scattered and absorbed more, leading to a higher measured absorbance than the actual absorbance.⚹◊How does the orientation of the cuvette affect the measured absorbance of $FeSCN^{2+}$ solution in a spectrophotometer?⍭ Generate me a similar question◊

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