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Question
Biology
Posted 2 months ago
Explain how absorption of UV light can be used to measure concentration of a protein?
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Answer from Sia
Posted 2 months ago
Answer
The absorption of UV light can be used to measure the concentration of a protein by applying the Beer-Lambert law, which relates absorbance to concentration.
Solution
a
Principle of UV absorption: Proteins absorb UV light primarily due to the presence of aromatic amino acids
b
Beer-Lambert Law: The law states that the absorbance (A) is directly proportional to the concentration (c) of the absorbing species and the path length (l) of the sample cell. The mathematical expression is A=ϵclA = \epsilon \cdot c \cdot l, where ϵ\epsilon is the molar absorptivity
c
Measurement and calculation: Measure the absorbance of the protein solution at 280 nm, where proteins typically show maximum absorption due to tryptophan and tyrosine residues. Use the measured absorbance and known path length to calculate the protein concentration using the Beer-Lambert Law
Key Concept
Beer-Lambert Law
Explanation
The Beer-Lambert Law is used to calculate the concentration of a protein solution based on its absorbance of UV light at a specific wavelength (typically 280 nm for proteins).

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