Figure 1 shows the chromatogram of an HPLC analysis of an environment water sample containing eight (8) compounds of interest possessing non-polar and mid-polar properties. Answer following questions related to the chromatogram.
Figure 1: A chromatogram of a mixture of different polarity in a packed HPLC column. The flow rate of mobile phase was . The column labelling denoted as .
i. What is the meaning of ' '? Recognize each of them.
ii. Suggest the most suitable type of HPLC stationary phase column for successful separation of these analytes.
iii. Explain the interaction between analyte and the suggested stationary phase column mentioned in d (ii).
iv. Comment on the overall separation criteria for the 8 analytes.
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