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Biology
Posted 3 months ago
How we calculate colonies in membrane filteration experiment
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Answer from Sia
Posted 3 months ago
Answer
To calculate colonies in a membrane filtration experiment, count the number of colonies on the filter, and then calculate the number of colonies per volume of sample filtered.
Solution
a
Count the colonies: After incubation, count the number of colonies that have grown on the membrane filter
b
Calculate the concentration: Use the formula Number of colonies×1Volume of sample filtered (in mL) \text{Number of colonies} \times \frac{1}{\text{Volume of sample filtered (in mL)}} to calculate the concentration of colonies per mL of the original sample
Key Concept
Colony Forming Units (CFU)
Explanation
CFU is a measure used to estimate the number of viable bacteria or fungal cells in a sample. In membrane filtration, colonies are counted to determine the CFU per volume of the original sample.

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