Question

Biology

Posted 5 months ago

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If we have 63 colonies on plate and water sample we used is
1.0 ml then what is answer in membrane filteration
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Answer from Sia

Posted 5 months ago

Answer

The concentration of bacteria in the water sample is 63,000 CFU/ml.

Solution

a

Calculate the number of colony-forming units (CFU) per milliliter of the original water sample: To find the concentration of bacteria in the water sample, you multiply the number of colonies by the reciprocal of the volume of the water sample that was filtered

b

Using the formula: $\text{CFU/ml} = \frac{\text{Number of colonies}}{\text{Volume of water sample in ml}}$

c

Substituting the given values into the formula: $\text{CFU/ml} = \frac{63 \text{ colonies}}{1.0 \text{ ml}} = 63 \text{ colonies/ml}$

d

Since the results are typically expressed in terms of CFU per 100 ml of water, we multiply the CFU/ml by 100: $\text{CFU/100 ml} = 63 \text{ colonies/ml} \times 100 = 6300 \text{ CFU/100 ml}$

e

However, in environmental microbiology, it is common to report the number of CFU per 100 ml as CFU/ml for standardization and comparison purposes. Therefore, we need to convert CFU/100 ml back to CFU/ml by multiplying by 10: $\text{CFU/ml} = 6300 \text{ CFU/100 ml} \times 10 = 63,000 \text{ CFU/ml}$

Key Concept

Membrane filtration technique is used to estimate the concentration of bacteria in a water sample by counting the number of colonies that grow on a plate and adjusting for the volume of water filtered.

Explanation

The number of colonies on the plate (63) represents the number of bacteria that were able to grow from the 1.0 ml water sample. To find the concentration in the original sample, we multiply the number of colonies by the reciprocal of the volume of the sample. The result is then standardized to CFU/ml, which in this case is 63,000 CFU/ml.

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