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Biology
Posted 6 months ago
In a harmonized microbial limits test, while performing suitability of methods test, a lab dechnician counted an average of 95 colonies on the internal control plates and on the plates with diluted product +10+10^{*} celsil he counted 68 cofonies. The technician in the lab is unsure as to Whether the product has passed or failed the suitability of methods test. How would you help the lab technician to make the right decision? Explain (2 marks)
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Answer from Sia
Posted 6 months ago
Answer
The product has failed the suitability of methods test.
Solution
a
Calculate the percentage recovery of the colonies on the plates with the diluted product: The percentage recovery is calculated using the formula Number of colonies on product plateNumber of colonies on control plate×100%\frac{\text{Number of colonies on product plate}}{\text{Number of colonies on control plate}} \times 100\%
b
Apply the acceptance criteria for the suitability of methods test: Typically, the recovery should be within 70-130% for the product to pass the test
Key Concept
Suitability of methods test in microbial limits testing
Explanation
The suitability of methods test ensures that the test method is capable of recovering microorganisms from the product at an acceptable level, usually within 70-130% of the control.

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