Suppose you performed a Sanger sequencing reaction using a radioactive primer on the template shown below. Draw the expected result. A a P a B 5' \square OH 8 a 3' \square - TTTCCCAAAGGG = \} mu DNA polymerase + dNTP + ddATP / ddCTP/ ddGTP / ddTTP Autoradiogram of single - stranded DNA Radioactively labeled electrophoresis C D dd I 7 |

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∻Answer∻ ⚹ The expected result is a series of bands on the autoradiogram, each corresponding to different lengths of DNA fragments terminated by ddNTPs. ⚹ ∻Solution∻ ‖ a ⋮ Template DNA: The given template DNA sequence is 5'-TTTCCCAAAGGG-3'. ‖ ‖ b ⋮ Primer Annealing: A radioactive primer will anneal to the 3' end of the template DNA. ‖ ‖ c ⋮ DNA Polymerization: DNA polymerase will extend the primer by adding dNTPs complementary to the template until a ddNTP is incorporated, which terminates the extension. ‖ ‖ d ⋮ ddNTP Incorporation: The ddNTPs (ddATP, ddCTP, ddGTP, ddTTP) lack a 3' hydroxyl group, preventing further extension once incorporated. ‖ ‖ e ⋮ Expected Fragments: The fragments generated will be: - 5'-ddA - 5'-ddCA - 5'-ddGCA - 5'-ddTGCA - 5'-ddATGCA - 5'-ddAATGCA - 5'-ddGAATGCA - 5'-ddCGAATGCA - 5'-ddCCGAATGCA - 5'-ddACCGAATGCA - 5'-ddAACCGAATGCA - 5'-ddGAACCGAATGCA ‖ ‖ f ⋮ Autoradiogram: The autoradiogram will show bands corresponding to the length of each terminated fragment, with the shortest fragment closest to the well and the longest fragment furthest from the well. ‖ ∻Key Concept∻ ⚹ Sanger sequencing is a method of DNA sequencing based on selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. ⚹ ∻Explanation∻ ⚹ In Sanger sequencing, the incorporation of a specific ddNTP terminates the DNA strand extension, resulting in fragments of varying lengths that can be separated by electrophoresis to determine the DNA sequence. ⚹◊What is the purpose of using a radioactive primer in a Sanger sequencing reaction?⍭ Generate me a similar question◊

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