The physician takes scrapings from a patient's arm. They tell you they want to perform a direct examination of the scrapings for the presence of fungus. What type of preparation should you do

Question

Accepted Answer

∻Answer∻ ⚹ To perform a direct examination of the scrapings for the presence of fungus, you should prepare a potassium hydroxide (KOH) mount. ⚹ ∻Solution∻ ‖ a ⋮ Collect the scrapings: Use a sterile scalpel or blade to gently scrape the affected area of the patient's skin to collect the sample. ‖ ‖ b ⋮ Place the sample on a slide: Transfer the collected scrapings onto a clean glass microscope slide. ‖ ‖ c ⋮ Add KOH solution: Add a drop of 10-20% potassium hydroxide (KOH) solution to the sample on the slide. ‖ ‖ d ⋮ Cover with a coverslip: Gently place a coverslip over the sample to spread the KOH solution evenly. ‖ ‖ e ⋮ Heat gently (optional): If necessary, gently heat the slide to speed up the clearing process, but avoid boiling. ‖ ‖ f ⋮ Examine under a microscope: Observe the slide under a microscope for the presence of fungal elements such as hyphae or spores. ‖ ∻Key Concept∻ ⚹ Potassium hydroxide (KOH) mount ⚹ ∻Explanation∻ ⚹ The KOH mount is a common preparation method used to detect fungal elements in clinical samples. KOH dissolves keratin and other cellular material, making it easier to visualize fungi under a microscope. ⚹◊What staining technique would you use to visualize the presence of fungus in the scrapings from the patient's arm?⍭ Generate me a similar question◊

Sia