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Question
Biology
Posted about 2 months ago
The physician takes scrapings from a patient's arm. They tell you they want to perform a direct examination of the scrapings for the presence of fungus. What type of preparation should you do
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Answer from Sia
Posted about 2 months ago
Answer
To perform a direct examination of the scrapings for the presence of fungus, you should prepare a potassium hydroxide (KOH) mount.
Solution
a
Collect the scrapings: Use a sterile scalpel or blade to gently scrape the affected area of the patient's skin to collect the sample
b
Place the sample on a slide: Transfer the collected scrapings onto a clean glass microscope slide
c
Add KOH solution: Add a drop of 10-20% potassium hydroxide (KOH) solution to the sample on the slide
d
Cover with a coverslip: Gently place a coverslip over the sample to spread the KOH solution evenly
e
Heat gently (optional): If necessary, gently heat the slide to speed up the clearing process, but avoid boiling
f
Examine under a microscope: Observe the slide under a microscope for the presence of fungal elements such as hyphae or spores
Key Concept
Potassium hydroxide (KOH) mount
Explanation
The KOH mount is a common preparation method used to detect fungal elements in clinical samples. KOH dissolves keratin and other cellular material, making it easier to visualize fungi under a microscope.

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