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Question
Biology
Posted 5 months ago
What is a major difference between DNA polymerase I and DNA polymerase II?
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Answer from Sia
Posted 5 months ago
Answer
The major difference between DNA polymerase I and DNA polymerase III is their primary functions and associated activities in DNA replication.
Solution
a
Primary function: DNA polymerase I is primarily involved in the removal of RNA primers from the DNA and replacing them with the appropriate nucleotides, whereas DNA polymerase III is the main enzyme responsible for DNA synthesis during replication
b
Associated activities: DNA polymerase I has exonuclease activity in both the 5' to 3' and 3' to 5' directions, which allows it to remove primers and fill in the gaps with DNA. DNA polymerase III, on the other hand, has high processivity and 3' to 5' exonuclease activity for proofreading but lacks 5' to 3' exonuclease activity
Key Concept
Functional distinction in DNA replication
Explanation
DNA polymerase I is involved in primer removal and gap filling, while DNA polymerase III is the primary enzyme for strand elongation and proofreading during DNA replication.

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