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Biology
Posted 6 months ago
What is gene expression testing in molecular diagnostics in biology
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Answer from Sia
Posted 6 months ago
Answer
Gene expression testing in molecular diagnostics is a technique used to measure the activity (the expression) of specific genes in a sample.
Solution
a
Gene expression testing involves extracting RNA from a sample, which is then converted into complementary DNA (cDNA) using reverse transcription
b
The cDNA is then amplified and quantified using techniques such as quantitative PCR (qPCR) or next-generation sequencing (NGS)
c
This process allows for the detection and quantification of specific gene transcripts, providing insights into gene activity and regulation
Key Concept
Gene expression testing
Explanation
Gene expression testing is crucial in molecular diagnostics as it helps in understanding the functional state of genes, which can be indicative of various diseases, including cancers and genetic disorders.
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