What is the best method for protein purification based on size, gel filtration, or SDS-PAGE?

Question

Accepted Answer

∻Answer∻ ⚹ Gel filtration chromatography is generally the best method for protein purification based on size. ⚹ ∻Solution∻ ‖ a ⋮ Gel filtration chromatography: This method, also known as size-exclusion chromatography, separates proteins based on their size by passing them through a column filled with porous beads. Larger proteins elute first because they cannot enter the pores, while smaller proteins elute later as they enter the pores and take a longer path. ‖ ‖ b ⋮ SDS-PAGE: This technique is primarily used for analyzing protein size rather than purification. Proteins are denatured and coated with SDS, giving them a uniform negative charge. They are then separated by electrophoresis based on their molecular weight. While SDS-PAGE is excellent for determining protein size, it is not typically used for purification due to its denaturing conditions. ‖ ∻Key Concept∻ ⚹ Gel filtration chromatography ⚹ ∻Explanation∻ ⚹ Gel filtration chromatography is preferred for protein purification based on size because it maintains the native state of proteins and effectively separates them based on their size without denaturing them. ⚹◊What is the principle behind separating proteins by size using gel filtration chromatography?⍭ Generate me a similar question◊

Sia