What is the biochemical effect of 𝑝-hydroxymercuribenzoate on the enzyme ATCase?

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∻Answer∻ ⚹ The biochemical effect of 𝑝-hydroxymercuribenzoate on the enzyme ATCase is that it inhibits the enzyme by binding to its cysteine residues, leading to a conformational change that reduces its activity. ⚹ ∻Solution∻ ‖ a ⋮ ATCase (Aspartate Transcarbamoylase) is an enzyme involved in the pyrimidine biosynthesis pathway. It catalyzes the reaction between aspartate and carbamoyl phosphate to form N-carbamoylaspartate. ‖‖ b ⋮ 𝑝-hydroxymercuribenzoate is a sulfhydryl reagent that specifically reacts with thiol groups (-SH) in cysteine residues of proteins. ‖‖ c ⋮ When 𝑝-hydroxymercuribenzoate binds to the cysteine residues in ATCase, it forms a covalent bond, leading to a conformational change in the enzyme's structure. ‖‖ d ⋮ This conformational change can inhibit the enzyme's activity by preventing the proper alignment of the active site or by altering the enzyme's ability to bind to its substrates. ‖ ∻Key Concept∻ ⚹ Inhibition of ATCase by 𝑝-hydroxymercuribenzoate ⚹ ∻Explanation∻ ⚹ 𝑝-hydroxymercuribenzoate inhibits ATCase by binding to its cysteine residues, causing a conformational change that reduces the enzyme's activity. ⚹◊How does p-hydroxymercuribenzoate affect the activity of the enzyme ATCase at a molecular level⍭ Generate me a similar question◊

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