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Question
Biology
Posted 8 months ago
Why use the soft agar overlay technique?
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Answer from Sia
Posted 8 months ago
Answer
The soft agar overlay technique is used to isolate and count bacteriophages (viruses that infect bacteria) by creating a double-layer agar plate.
Solution
a
Soft agar overlay: This technique involves pouring a mixture of bacteria and bacteriophages into a soft agar layer, which is then poured over a solid agar plate
b
Plaque formation: Bacteriophages infect the bacteria in the soft agar, leading to the formation of clear zones called plaques, which represent areas where the bacteria have been lysed by the phages
Key Concept
Soft agar overlay technique
Explanation
This technique is essential for isolating and counting bacteriophages, as it allows for the visualization of plaques, which indicate phage activity.

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