Would the plaque assay be useful for calculating lambda phage titers? Explain your reasoning.

Question

Accepted Answer

∻Answer∻ ⚹ Yes, the plaque assay would be useful for calculating lambda phage titers. ⚹ ∻Solution∻ ‖ a ⋮ Principle of Plaque Assay: The plaque assay is a method used to measure the number of viral particles present in a sample. ‖ ‖ b ⋮ Application to Lambda Phage: Lambda phage, a bacteriophage, forms clear plaques on a lawn of bacteria, which can be counted to determine the viral titer. ‖ ‖ c ⋮ Calculation of Titer: The number of plaques counted is used to calculate the titer of the phage suspension by using the formula $ ext{Titer (PFU/ml)} = \frac{ ext{Number of plaques}}{ ext{Volume of phage suspension plated (ml)}} imes ext{Dilution factor} $. ‖ ∻Key Concept∻ ⚹ Plaque assay for viral titer determination ⚹ ∻Explanation∻ ⚹ The plaque assay is a quantitative method that allows for the determination of the number of infectious virus particles in a sample, which is applicable to lambda phage. ⚹◊How does the plaque assay differentiate between live and dead lambda phages?⍭ Generate me a similar question◊

Sia