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You have a small culture with a volume of $10 \mathrm{~mL}$ and a cell density of $1 \times 10^{6}$ cells $/ \mathrm{mL}$. You want to scale this up to a larger volume of $1 L$ while maintaining the same cell density. How many cells in total do you need, and how would you prepare this larger culture?
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Answer from Sia

Posted 6 months ago

1 Answer

You need 1 x 10^9 cells in total for the larger culture.

Solution

a

Calculate the total number of cells in the small culture: $10 \, \text{mL} \times 1 \times 10^6 \, \text{cells/mL} = 1 \times 10^7 \, \text{cells}$

b

Determine the total number of cells needed for the larger culture: $1 \, \text{L} = 1000 \, \text{mL}$, so $1000 \, \text{mL} \times 1 \times 10^6 \, \text{cells/mL} = 1 \times 10^9 \, \text{cells}$

c

To prepare the larger culture, you would need to add enough cells to reach the desired cell density of $1 \times 10^6 \, \text{cells/mL}$ in 1 L of culture medium. This can be done by scaling up the initial culture or by concentrating cells from a larger volume

Key Concept

Scaling up cell culture

Explanation

To scale up a cell culture while maintaining cell density, calculate the total number of cells needed for the new volume and adjust the culture conditions accordingly.

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