You have four different proteins that you want to separate from each other.
Protein A has a pl of 6.8 and a molecular weight of 66,000 , Protein has a pl of 7.0 and a molecular weight to 125,000 , Protein has a pl of 6.1 and a molecular weight of 50,000 and Protein D has a pl of 10 and a molecular weight of 11,000 . What chromatography technique would you use and why? Can you predict the elution order
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