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Biology
Posted 7 months ago
describe a negative control to show that the dna has come from you and not environment
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Answer from Sia
Posted 7 months ago
Answer
To describe a negative control to show that the DNA has come from you and not the environment, follow these steps:
Solution
a
Collect a sample from a known source: Obtain a DNA sample from yourself, ensuring that the collection process is sterile to avoid contamination
b
Prepare a negative control: Use a sterile swab or collection tool to collect a sample from an area where no DNA is expected, such as a clean, sterile surface
c
Perform DNA extraction: Extract DNA from both the known source (yourself) and the negative control sample using the same protocol
d
Run PCR amplification: Amplify the DNA from both samples using Polymerase Chain Reaction (PCR) with specific primers
e
Analyze the results: Compare the PCR results. The sample from yourself should show amplified DNA, while the negative control should show no amplification, indicating no contamination from the environment
Key Concept
Negative control in DNA experiments
Explanation
A negative control is used to ensure that the DNA detected in an experiment is not due to contamination from the environment. By comparing the results from a known source and a sterile sample, one can confirm the origin of the DNA.

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