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Posted 2 months ago
how does denaturation and renaturation contribute to PCR and DNA replication
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Answer from Sia
Posted 2 months ago
Denaturation and renaturation are crucial steps in PCR that facilitate the replication of DNA.
Denaturation in PCR: This is the process of heating the DNA to around 95°C to break the hydrogen bonds between the two strands, resulting in single-stranded DNA molecules
Renaturation in PCR: This refers to the cooling of the PCR mixture to allow primers to anneal (bind) to the complementary sequences on the single-stranded DNA templates
Extension in PCR: Following renaturation, the temperature is raised to the optimal temperature for DNA polymerase to synthesize new DNA strands by adding nucleotides to the primers
Key Concept
Denaturation and renaturation are essential for separating DNA strands and allowing primer binding, respectively, which are necessary for DNA amplification in PCR.
Denaturation provides single-stranded templates for the primers to bind during renaturation, which is followed by the synthesis of new DNA strands, thereby duplicating the DNA sequence of interest.

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