Question

Biology

Posted 4 months ago

`using the standard curve that you constructed, determine how far the following proteins would migrate if electrophoresed on your gel SDS-PAGE gel`

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Answer from Sia

Posted 4 months ago

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Sure, let's determine how far the proteins would migrate on an SDS-PAGE gel using the standard curve you constructed.
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Answer

The migration distance of the proteins on the SDS-PAGE gel is determined based on their molecular weights and the standard curve.

Solution

a

Identify the molecular weights of the proteins you are analyzing. For example, let's assume the proteins have molecular weights of 25 kDa, 50 kDa, and 75 kDa

b

Use the standard curve equation, which is typically in the form of $y = mx + b$, where $y$ is the migration distance, $m$ is the slope, $x$ is the log of the molecular weight, and $b$ is the y-intercept

c

Calculate the log of the molecular weights:
$\log(25) = 1.398, \quad \log(50) = 1.699, \quad \log(75) = 1.875$

d

Substitute these values into the standard curve equation to find the migration distances. For example, if the standard curve equation is $y = -2.5x + 10$, then:
$\text{For 25 kDa: } y = -2.5(1.398) + 10 = 6.505$
$\text{For 50 kDa: } y = -2.5(1.699) + 10 = 5.253$
$\text{For 75 kDa: } y = -2.5(1.875) + 10 = 5.312$

Key Concept

Standard curve in SDS-PAGE

Explanation

The standard curve relates the migration distance of proteins to their molecular weights, allowing us to determine how far proteins will migrate based on their molecular weights.

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