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Question
Biology
Posted 4 months ago
using the standard curve that you constructed, determine how far the following proteins would migrate if electrophoresed on your gel SDS-PAGE gel
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Answer from Sia
Posted 4 months ago
Sure, let's determine how far the proteins would migrate on an SDS-PAGE gel using the standard curve you constructed.

Answer
The migration distance of the proteins on the SDS-PAGE gel is determined based on their molecular weights and the standard curve.
Solution
a
Identify the molecular weights of the proteins you are analyzing. For example, let's assume the proteins have molecular weights of 25 kDa, 50 kDa, and 75 kDa
b
Use the standard curve equation, which is typically in the form of y=mx+by = mx + b, where yy is the migration distance, mm is the slope, xx is the log of the molecular weight, and bb is the y-intercept
c
Calculate the log of the molecular weights: log(25)=1.398,log(50)=1.699,log(75)=1.875 \log(25) = 1.398, \quad \log(50) = 1.699, \quad \log(75) = 1.875
d
Substitute these values into the standard curve equation to find the migration distances. For example, if the standard curve equation is y=2.5x+10y = -2.5x + 10, then: For 25 kDa: y=2.5(1.398)+10=6.505 \text{For 25 kDa: } y = -2.5(1.398) + 10 = 6.505 For 50 kDa: y=2.5(1.699)+10=5.253 \text{For 50 kDa: } y = -2.5(1.699) + 10 = 5.253 For 75 kDa: y=2.5(1.875)+10=5.312 \text{For 75 kDa: } y = -2.5(1.875) + 10 = 5.312
Key Concept
Standard curve in SDS-PAGE
Explanation
The standard curve relates the migration distance of proteins to their molecular weights, allowing us to determine how far proteins will migrate based on their molecular weights.

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