Explain the Perl's stain for FNA sample of liver disease? Explain the IHC stain for FNA sample of lymph node?

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∻1 Answer∻ ⚹ Perl's stain is used to detect iron deposits in liver disease. ⚹ ∻1 Solution∻ ‖ a ⋮ Preparation of FNA sample: The fine needle aspiration (FNA) sample from the liver is spread on a slide and fixed. ‖ ‖ b ⋮ Application of Perl's Prussian blue stain: The fixed slide is treated with a mixture of hydrochloric acid and potassium ferrocyanide. ‖ ‖ c ⋮ Visualization of iron deposits: Iron deposits in the tissue react with the staining solution to form blue or purple Prussian blue complexes, which are then visualized under a microscope. ‖ ∻Key Concept∻ ⚹ Perl's Prussian blue reaction ⚹ ∻Explanation∻ ⚹ Perl's stain detects iron by forming Prussian blue complexes with iron deposits, indicating hemosiderosis or other iron accumulation disorders in liver disease. ⚹ ∻2 Answer∻ ⚹ IHC stain is used to detect specific antigens in lymph node FNA samples. ⚹ ∻2 Solution∻ ‖ a ⋮ Preparation of FNA sample: The fine needle aspiration (FNA) sample from the lymph node is spread on a slide and fixed. ‖ ‖ b ⋮ Application of primary antibody: The fixed slide is treated with a primary antibody specific to an antigen of interest. ‖ ‖ c ⋮ Binding of secondary antibody: A secondary antibody, which is linked to an enzyme or a fluorescent dye, binds to the primary antibody. ‖ ‖ d ⋮ Visualization of antigen-antibody complexes: The slide is treated with a substrate that reacts with the enzyme or is observed under a fluorescence microscope, revealing the location of the antigen. ‖ ∻Key Concept∻ ⚹ Antigen-antibody specificity ⚹ ∻Explanation∻ ⚹ IHC staining uses the specificity of antigen-antibody interactions to detect and visualize specific proteins within cells in lymph node FNA samples, aiding in diagnosis. ⚹◊How does Perl's stain specifically target iron deposits in FNA samples of liver disease?⍭ Generate me a similar question◊

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