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Biology
Posted 2 months ago
Explain the Perl's stain for FNA sample of liver disease? Explain the IHC stain for FNA sample of lymph node?
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Answer from Sia
Posted 2 months ago
1 Answer
Perl's stain is used to detect iron deposits in liver disease.
1 Solution
a
Preparation of FNA sample: The fine needle aspiration (FNA) sample from the liver is spread on a slide and fixed
b
Application of Perl's Prussian blue stain: The fixed slide is treated with a mixture of hydrochloric acid and potassium ferrocyanide
c
Visualization of iron deposits: Iron deposits in the tissue react with the staining solution to form blue or purple Prussian blue complexes, which are then visualized under a microscope
Key Concept
Perl's Prussian blue reaction
Explanation
Perl's stain detects iron by forming Prussian blue complexes with iron deposits, indicating hemosiderosis or other iron accumulation disorders in liver disease.
2 Answer
IHC stain is used to detect specific antigens in lymph node FNA samples.
2 Solution
a
Preparation of FNA sample: The fine needle aspiration (FNA) sample from the lymph node is spread on a slide and fixed
b
Application of primary antibody: The fixed slide is treated with a primary antibody specific to an antigen of interest
c
Binding of secondary antibody: A secondary antibody, which is linked to an enzyme or a fluorescent dye, binds to the primary antibody
d
Visualization of antigen-antibody complexes: The slide is treated with a substrate that reacts with the enzyme or is observed under a fluorescence microscope, revealing the location of the antigen
Key Concept
Antigen-antibody specificity
Explanation
IHC staining uses the specificity of antigen-antibody interactions to detect and visualize specific proteins within cells in lymph node FNA samples, aiding in diagnosis.

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