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Question
Biology
Posted 5 months ago
How is the DdeI restriction enzyme useful in the PCR lab we did?
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Answer from Sia
Posted 5 months ago
Answer
The DdeI restriction enzyme is useful in PCR labs for DNA fragment analysis and cloning.
Solution
a
Digestion of PCR products: DdeI can be used to digest PCR products to check for the presence or absence of specific mutations or polymorphisms
b
Cloning: DdeI can create sticky ends on DNA fragments that can be ligated into vectors with compatible ends for cloning purposes
Key Concept
DdeI restriction enzyme's role in PCR labs
Explanation
DdeI is used to cut DNA at specific sequences, which is helpful for analyzing PCR products and preparing DNA

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