There are different types of PAGE electrophoresis. Explain what the basis of protein separation is for the
following techniques.

Question

There are different types of PAGE electrophoresis. Explain what the basis of protein separation is for the
following techniques.

Accepted Answer

Sure, let's break down the basis of protein separation for different types of PAGE electrophoresis techniques. ∻Solution∻ ‖ a ⋮ SDS-PAGE: This technique separates proteins based on their molecular weight. Proteins are denatured and coated with sodium dodecyl sulfate (SDS), which imparts a uniform negative charge. When an electric field is applied, proteins migrate towards the positive electrode, with smaller proteins moving faster through the gel matrix. ‖ ‖ b ⋮ Native PAGE: Unlike SDS-PAGE, Native PAGE separates proteins based on their native charge, size, and shape. Proteins are not denatured, so their tertiary and quaternary structures are preserved. This allows for the separation of proteins in their functional form. ‖ ‖ c ⋮ Isoelectric Focusing (IEF): This technique separates proteins based on their isoelectric point (pI). Proteins are subjected to a pH gradient in a gel, and they migrate to the point where their net charge is zero (their pI). ‖ ∻Answer∻ ⚹ SDS-PAGE separates proteins by molecular weight, Native PAGE by native charge, size, and shape, and IEF by isoelectric point. ⚹ ∻Key Concept∻ ⚹ Protein separation in PAGE electrophoresis ⚹ ∻Explanation∻ ⚹ Different PAGE techniques separate proteins based on distinct properties: SDS-PAGE by molecular weight, Native PAGE by native charge, size, and shape, and IEF by isoelectric point. ⚹◊What factors influence protein migration in PAGE electrophoresis?⍭ Generate me a similar question◊

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