Why would plate b with IX and pLZDonorGuide with Cas9 have no growth?

Question

Accepted Answer

∻Answer∻ ⚹ The absence of growth on plate b with IX and pLZDonorGuide with Cas9 is likely due to the successful cutting of the target DNA by Cas9, leading to cell death or the inability to grow. ⚹ ∻Solution∻ ‖ a ⋮ Cas9 ⋮ Cas9 is an endonuclease that introduces double-strand breaks (DSBs) in DNA at specific locations guided by the pLZDonorGuide RNA. ‖ ‖ b ⋮ DNA Damage ⋮ The DSBs created by Cas9 can be lethal to cells if not repaired properly, leading to cell death or growth inhibition. ‖ ‖ c ⋮ No Repair ⋮ If the cells on plate b do not have the necessary repair mechanisms or donor DNA to repair the DSBs, they will not survive or grow. ‖ ∻Key Concept∻ ⚹ Cas9-induced DNA double-strand breaks ⚹ ∻Explanation∻ ⚹ Cas9, guided by the pLZDonorGuide RNA, introduces double-strand breaks in the DNA. If these breaks are not repaired, the cells cannot survive or grow, leading to no growth on the plate. ⚹◊What is the role of Cas9 in the growth inhibition of plate b with IX and pLZDonorGuide⍭ Generate me a similar question◊

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