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Question
Biology
Posted 7 months ago
what is the reason of lysis buffer
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Answer from Sia
Posted 7 months ago
Answer
The reason for using a lysis buffer is to break open cells to release their contents for further analysis.
Solution
a
Cell Disruption: Lysis buffers contain detergents that disrupt the cell membrane, allowing the release of cellular contents
b
Protein Denaturation: They often contain agents that denature proteins, making them easier to study
c
pH Stabilization: Lysis buffers maintain a stable pH to protect the integrity of the released biomolecules
Key Concept
Lysis Buffer Function
Explanation
A lysis buffer is used to break open cells, denature proteins, and stabilize pH, facilitating the extraction and analysis of cellular components.
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