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Biology
Posted 8 months ago
why heat inactivated fbs is used in cell culture
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Answer from Sia
Posted 8 months ago
Answer
Heat-inactivated FBS is used in cell culture to deactivate complement proteins that can damage cells and to reduce the risk of mycoplasma contamination.
Solution
a
Complement inactivation: Heat inactivation deactivates complement proteins in FBS, which could otherwise lyse the cultured cells
b
Mycoplasma risk reduction: Heat treatment can also destroy mycoplasma and other potential contaminants that might be present in the serum
Key Concept
Heat inactivation of FBS is crucial for protecting cultured cells from complement-mediated lysis and reducing contamination risks.
Explanation
Heat inactivation at 56°C for 30 minutes is a standard procedure to deactivate complement proteins and reduce contamination in FBS used for cell culture.
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