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Question
Biology
Posted 4 months ago
Which biochemical test using that PH indicator turns alkaline when the organism is negative for that test
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Answer from Sia
Posted 4 months ago
Answer
The biochemical test that uses a pH indicator which turns alkaline when the organism is negative for that test is the urease test.
Solution
a
Urease Test: This test is used to identify organisms that produce the enzyme urease
b
pH Indicator: Phenol red is commonly used as the pH indicator in the urease test
c
Negative Result: If the organism does not produce urease, the medium remains or turns alkaline, and the pH indicator will reflect this by either not changing color or turning a deep pink/red
Key Concept
Urease Test and pH Indicator
Explanation
The urease test uses phenol red as a pH indicator to detect the presence of urease by observing the color change of the medium, which turns alkaline and changes to a deep pink/red when the test is negative.

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